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The races start and finish in downtown Louisville. They follow the same route to the west side, back to downtown, and through Old Louisville and the University of Louisville campus to Churchill Downs, where they take a trip around the track in the infield before splitting after exiting the racetrack. The MiniMarathon (in fact a half-marathon) returns directly to downtown. The Marathon heads toward Iroquois Park in the south end, takes a loop around that park, and returns to downtown after taking a detour into the Highlands.
The Falls of the Ohio (which in their natural state were more a series of large rapids) were once a major barrier to navigation on the Ohio River. Louisville catered to steamboat passengers who spent the night before changing boats, and even more significantly earned considerable income from overland portaging of cargo to bypass the rapids. The construction of a bypass canal, including the first set of locks on the river, in 1830 changed all of that. The current structure dates to 1961. There's usually a lot of barge traffic on the Ohio (though it slows down in winter once the upper reaches of the river in Pennsylvania begin to freeze), so you normally won't have to wait long at the visitor center to see the locks in action.
Sprawling, hilly woodland area that includes numerous hiking trails, lakes and streams, camping areas, and other attractions. It was initially created as a World War II memorial and has been gradually expanded on a somewhat ad-hoc basis ever since, as a result there are parcels of private property seemingly fairly deep into the forest. They are mostly well marked so don't cross fences or clearly marked boundary lines.
Briefly open as a museum, it's now closed to the public. Serious fans may still enjoy stopping by and seeing the little pink house where The Greatest grew up. Do it in the daytime - it's not the greatest neighborhood after dark. For a serious, comprehensive museum of Ali's life and career, see the Ali Center downtown.
Golf Course/Pro Shop.
Home of the legendary Louisville Slugger baseball bat. Full tours include a visit to the factory where wooden bats are made (or a bat-making demonstration if production is not scheduled). The museum is easily recognizable by the six-story-tall steel baseball bat at the front entrance.